Discovering Organoids: Establishment, Culture & Manipulation

Discovering Organoids: Establishment, Culture & Manipulation

11-12 September 2017

Homerton College, University of Cambridge, UK

This course has now passed

Adult intestinal stem cells can give rise to in vitro long-term self-renewing mini gut structures termed “Intestinal Organoids”. These organoids resemble the epithelial structure of the intestine, where LGR5+ stem cells, located in crypts, represent a long term self-renewing population that gives rise to all cell types of the intestinal epithelium. Due to the long term self-renewing potential of LGR5+ stem cells, these mini-gut structures can be mechanically dissociated and passaged indefinitely allowing scientists to study various aspects of intestinal adult stem cell biology in vitro. In recent years a multitude of tissues has been shown to harbour adult stem cell populations that can give rise to long term in vitro organoid cultures.

 

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Therefore, this emerging technology opens the doors for scientists of various disciplines to identify the underlying causes for tissue specific diseases, pathways involved in stem cell maintenance and injury mediated stem cell response as well as cancer formation.

In this course’s practical sessions, participants will learn how to successfully establish mouse INTESTINAL or LUNG organoid cultures from primary mouse tissue samples.

 

PROGRAMME HERE

POSTER HERE

REGISTRATION is now closed

 

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INSTRUCTORS

Dr Bon-Kyoung Koo
WT-MRC Cambridge Stem Cell Institute

Dr Joo-Hyeon Lee
WT-MRC Cambridge Stem Cell Institute

Miss Amanda Andersson-Rolf
WT-MRC Cambridge Stem Cell Institute

Dr Jinwook Choi
WT-MRC Cambridge Stem Cell Institute

Dr Julie Watson
WT-MRC Cambridge Stem Cell Institute

Miss Catherine Dabrowska
WT-MRC Cambridge Stem Cell Institute

 

KEYNOTE

Dr Madeline Lancaster
MRC LMB University of Cambridge

Watch Dr Lancaster’s TED talk : Growing mini brains to discover what makes us human HERE

MORE ABOUT THE TEAM

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Previous ORGANOID course video:

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Disclaimer: CamBioScience reserves the right to make changes to the content of the workshop at any time. If for any reason an instructor becomes unavailable for the period of the workshop, CamBioScience may appoint a qualified replacement at their discretion. CamBioScience will make all reasonable efforts to inform all registered participants by e-mail about any changes made to the programme. CamBioScience shall not be responsible for any errors, omissions, or inaccuracies in the programme and accepts no liability whatsoever for any loss or damage to the participant howsoever arising. It is the participants responsibility to take care of all travel and accommodation arrangements to ensure participation at the workshop. Whilst we will use our reasonable endeavours to supply the services, we shall not be responsible for any failure to provide services or any unavailability. The client must inform CamBioScience by e-mail or in writing within 2 weeks post confirmed registration, but at the latest 2 weeks before the beginning of the workshop, about any cancellation of the course bookings. If the client fails to do so within the requested period post confirmation of registration and if no other applicant can fill the resulting empty slot in the workshop, the client will be charged in full for the initially accepted service.
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