Modern PCR & NGS methods of Clinical Relevance

Next Generation Sequencing (NGS) technology can allow unprecedented, gigabase-length sequencing of genomic DNA per run. NGS has rapidly become an important part of clinical molecular diagnostics. Importantly, PCR techniques play an integral role in targeted NGS sequencing. PCR allows for the generation of multiple NGS libraries and the sequencing of multiple targeted regions simultaneously.

On this course, you will learn to:

1. Obtain a deeper understanding of protocol development

2. Understand the digital PCR assay design

3. Gain further understanding of NGS Applications

4. Build a NGS Library Prep Workflow

5. Be able to perform NGS Data Analysis

• THEORY

• PRACTICAL

• NETWORKING

Participants will learn best practices for qPCR, dPCR, and NGS. The first day will be devoted to a survey of quantitative and digital PCR best practices and workflows with relevant lab exercises. Days two and three offer a deep dive into Next-Generation Sequencing library preparation. All the way from the basics of sequencing to the rigors of data analysis and beyond, including the power of personalized medicine.

Participants will experience multiple laboratory practicals. The practical sessions will begin with harvesting genomic DNA and end with purifying target sequence fragments via magnetic beads.

The course provides plenty of opportunities for networking with the instructors and other participants. Get answers and solutions to your individual research queries and find new collaborations.